电子邮件：lilitu@mail.hzau.edu.cn

办公地址：作物遗传改良国家重点实验室B404

涂礼莉 女，1997年考入yl6809永利yl6809永利作物遗传育种专业学习。2001-2007年在yl6809永利作物遗传改良国家重点实验室进行硕博阶段学习和研究，方向为棉花分子生物学。毕业后留校任教并继续在作物遗传改良国家重点实验室进行棉花纤维发育机制方面的研究。

棉花是关系到国计民生的重要经济作物，常年种植面积5000-6000万亩，年产棉纤维600万吨，产值1000多亿元。棉花纤维是种子表皮分化的单细胞，其极端伸长，细胞长相对于细胞直径可达到3000倍，而且成熟棉花纤维的纤维素含量>90%。研究纤维发育基因调控网络，有助于解析细胞分化命运决定、细胞膨胀伸长机制和纤维素沉积等重大基础理论问题。

我长期坚持利用海岛棉优质基因改良陆地棉品质的育种目标，克隆并验证了大量功能基因，描绘了优质纤维形成的调控网络，创制了优质棉花种质；从全基因组水平解析纤维发育机制，并鉴定优质纤维相关QTL，为基于棉花基因组育种打下了坚实的理论基础。

    目前关注2个科学问题。1. 以纤维细胞起始分化为例来阐释细胞命运决定。拟采用的研究手段包括：胚珠外珠被单细胞测序;纤维起始分化期646个转录因子Crispr突变体库；纤维起始分化期转录因子酵母双杂库；转录因子原位Chip-seq。2. 纤维素合成的机理及调控。拟采用的研究手段包括：冷冻电镜解析纤维素合酶CesA复合物结构；纤维素合酶Crispr突变体库；纤维次生壁合成372个转录因子Crispr突变体库；纤维次生壁合成相关转录因子酵母双杂库；转录因子原位Chip-seq。

在研

1. 国家自然科学基金面上项目：棉花纤维素合酶CesA的Cryo-EM结构和功能解析 （2022.01-2025.12），32172071，主持

2. 国家自然科学基金重点项目：棉花优质纤维基因关联位点的高通量验证及基因调控网络构建（2019.01-2023.12），31830062，参加

已结题

1. 国家自然科学基金面上项目：细胞壁蛋白GbEXPATR结构解析及棉纤维细胞壁属性与纤维品质相关性探究（2017.1-2020.12），31671739，主持

2. 湖北省杰青，棉花纤维细胞分化起始机制解析（2018.1-2020.12），2018CFA034，主持

3. 转基因重大专项子课题，优质纤维转基因棉花新品种培育（2016.1-2020.12），2016ZX08005-003-004，主持

4. 重点研发项目子课题， GbTCP改良棉花纤维品质的机理研究（2016.7-2020.12），2016YFD0100505-05，主持

5. 国家自然科学基金面上项目：海岛棉纤维伸长相关expansin基因的功能验证及启动子分析（2009.1-2011.12），30871560，主持

6. 国家自然科学基金面上项目：棉花纤维发育不同时期特异/优势启动子的克隆与验证（2011.1-2013.12），31071465，主持

7. 国家自然科学基金重点项目：钙信号及活性氧调控棉花纤维伸长及品质形成机理研究（2013.01—2017.12）31230056，参加

8. 国家自然科学基金面上项目：类黄酮影响棉花纤维发育机理及棕色纤维色素形成探究 （2015.1-2018.12），31471540，主持

9. 新教师基金: 海岛棉纤维发育早期特异启动子PGbPDF的克隆与分析（2009.1-2011.12）主持

10. 转基因专项子课题：转基因新技术新方法研究（2011.7-2014.12），2013ZX08010001-006，主持

11. 863：海岛棉优质基因标记开发及高产抗病分子标记及新品种培育（2012.1-2015.12），2012AA101108-3，参加

12. 武汉市晨光计划：海岛棉优质纤维基因的功能验证及在陆地棉中的应用（2011.7-2012.12）主持

1. 丰胜求，涂礼莉，闵玲，朱龙付，谢国生，李强，李海霞，院级“课程思政”示范建设项目“植物生理生化实验”，2021

2. 朱龙付，丰胜求，曾汉来，吴高兵，涂礼莉，国际级一流本科课程《基础生物化学》，2020

3. 涂礼莉，《基础生物化学》获yl6809永利2018年度教学质量优秀一等奖

发明专利

1. 张献龙，朱龙付，涂礼莉等等，“从棉花组织中抽提RNA的方法”2006获国家发明专利。专利号：ZL200410060637.6            

2. 涂礼莉，李阳，张献龙，朱龙付，邓锋林，“两个棉花纤维伸长期优势表达的启动子及应用”专利授权号： ZL201010582387.8

3. 张献龙，谭家福，涂礼莉，朱龙付，邓锋林，“利用棉花基因GbF3H改变花瓣颜色”，专利申请号：ZL201010582448.0    

4. 张献龙，邓锋林，涂礼莉，朱龙付，谭家福，“两个棉花纤维发育起始优势表达的强启动子及其应用”，专利授权号：ZL201010582335.0

5. 张献龙，李阳，涂礼莉, 袁道军：“棉花细胞壁伸展蛋白基因GbEXPATR 及应用”，专利授权号：ZL201410073300.2

6. 张献龙，韩杰，谭家福，涂礼莉：“一种棉花Phytosulfokine前体基因GhPSKP及制备方法和应用”，专利授权号：ZL 201210260715.1

7. 涂礼莉：胡海燕，谭家福，张献龙：“一种纤维伸长期优势表达的启动子及制备方法和应用”，专利授权号：ZL 201210288775.4

8. 金双侠，涂礼莉，张献龙，“一种提高棉花再生及转化效率的方法及应用”，专利授权号：ZL 2015108336180

主要奖励

1. 张献龙, 朱龙付, 涂礼莉, 聂以春, 王茂军, 郭小平, 金双侠, 林忠旭, 杨国正, 杨细燕, 闵玲, 袁道军, “yl6809永利棉花遗传改良创新团队”， 2021年获神农奖优秀创新团队奖

2. 张献龙，王茂军，涂礼莉，林忠旭，袁道军，“棉花纤维发育与品质形成生物学”， 2020年获湖北省自然科学奖一等奖

3. 张献龙，朱龙付，闵玲，杨细燕，袁道军，涂礼莉，金双侠, “棉花抗黄萎病的信号调控网络研究”, 获2020年教育部高等学校科学研究优秀成果奖（科学技术）自然科学奖一等奖

4. 张献龙，林忠旭，朱龙付，涂礼莉，金双侠，郭小平，“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖          

5. 张献龙，聂以春，朱龙付，林忠旭，郭小平，卢怀玉，杨国正，余宏章，涂礼莉，“强优势多抗杂交棉新品种“华杂棉H318”的选育与应用”获2012年湖北省科技进步一等奖    

6. 张献龙，杨细燕，金双侠，孙玉强，吴家和，涂礼莉，朱龙付，聂以春，郭小平，林忠旭,“棉花体细胞离体遗传操作技术与控制理论”获2012年高等学校科学研究优秀成果奖（科学技术）自然科学二等奖    

7.张献龙，聂以春，朱龙付，郭小平，卢怀玉，刘立清，林忠旭，涂礼莉，杨国正，金双侠，杨细燕，邹勇，余宏章，袁道军，“棉花种质创新及强优势杂交棉新品种选育与应用” 2013年国家科技进步二等奖

2022年

1. Pengcheng Wang, Kai Guo, Qian Su, Jinwu Deng, Xianlong Zhang, Lili Tu*. Histone ubiquitination controls organ size in cotton (Gossypium hirsutum), Plant J, 2022 https://doi.org/10.1111/tpj.15716

2021年

1. Zhengxiu Ye, Lu Qiao, Xiangyin Luo, Xinyuan Chen, Xianlong Zhang, Lili Tu*. Genome-wide identification of cotton GRAM family proteins reveals that GRAM31 regulates fiber length, J Exp Bot, 2021 , 72(7), 2477-2490

2. Xiangnan Zhang, Yuan Xue, Zeyuan Guan, Chen Zhou, Yangfan Nie, She Men, Qiang Wang, Cuicui Shen, Delin Zhang, Shuangxia Jin, Lili Tu*, Ping Yin*, Xianlong Zhang*. Structural insights into homotrimeric assembly of cellulose synthase CesA7 from Gossypium hirsutum, Plant Biotechnol J, 2021, 19(8):1579-1587

2020年

1. Zhonghua Li, Pengcheng Wang, Chunyuan You, Jiwen Yu, Xiangnan Zhang, Feilin Yan, Zhengxiu Ye, Chao Shen, Baoqi Li, Kai Guo, Nian Liu, Gregory N. Thyssen, David D. Fang, Keith Lindsey, Xianlong Zhang, Maojun Wang*，Lili Tu*. Combined GWAS and eQTL analysis uncovers a genetic regulatory network orchestrating the initiation of secondary cell wall development in cotton. New Phytol, 2020, 226(6):1738-1752

2. Nian Liu, Shuchang Wu, Zhonghua Li, Anam Qadir Khan, Haiyan Hu, Xianlong Zhang, Lili Tu*, Repression of microRNA 160 results in retarded seed integument growth and smaller final seed size in cotton. Crop J, 2020, 8(4): 602-612  

3.Kai Guo, Zhonghua Li, Hanxue Tian, Xueqiong Du, Zhen Liu, Hui Huang, Pengcheng Wang, Zhengxiu Ye, Xianlong Zhang, Lili Tu*，Cytosolic ascorbate peroxidases (cAPXs) plays a critical role in photosynthesis by modulating reactive oxygen species (ROS) level in stomatal guard cell. Front Plant Sci. DOI: 10.3389/fpls.2020.00446

2019年

1. Maojun Wang^#, Lili Tu^#, Daojun Yuan^#, De Zhu, Chao Shen, Jianying Li, Fuyan Liu, Liuling Pei, Pengcheng Wang, Guannan Zhao, Zhengxiu Ye, Hui Huang, Feilin Yan, Yizan Ma, Lin Zhang, Min Liu, Jiaqi You, Yicheng Yang, Zhenping Liu, Fan Huang, Baoqi Li, Ping Qiu, Qinghua Zhang, Longfu Zhu, Shuangxia Jin, Xiyan Yang, Ling Min, Guoliang Li, Ling-Ling Chen, Hongkun Zheng, Keith Lindsey*, Zhongxu Lin*, Joshua A. Udall* and Xianlong Zhang*. Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense. Nat Genet. 2019 Feb;51(2):224-229

2018年

2. Haiyan Hu, MaojunWang, Yuanhao Ding, Sitao Zhu, Guannan Zhao, Lili Tu*, Xianlong Zhang. Transcriptomic repertoires depict the initiation of lint and fuzz fibers in cotton (Gossypium hirsutum L.), Plant Biotechnol J, 2018, 16(5):1002-1012

3. Maojun Wang, Pengcheng Wang, Fan Liang, Zhengxiu Ye, Jianying Li, Chao Shen, Liuling Pei, Feng Wang, Jiang Hu, Lili Tu, Keith Lindsey, Daohua He *, Xianlong Zhang *. A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation, New Phytol, 2018, 217(1):163-178

4. Maojun Wang, Pengcheng Wang, Min Lin, Zhengxiu Ye, Guoliang Li, Lili Tu, Chao Shen, Jianying Li, Qingyong Yang, Xianlong Zhang. Evolutionary dynamics of 3D genome architecture following polyploidization in cotton, Nat Plants, 2018, 4(2):90-97

2017年

5. Kai Guo, Lili Tu, Yonghui He, Jinwu Deng, Maojun Wang, Hui Huang, Zhonghua Li, Xianlong Zhang. Interaction between calcium and potassium modulates elongation rate in cotton fiber cells, J Exp Bot, 2017, 68(18):5161-5175

6. Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, Jianying Li, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, Cong Huang, Shuangxia Jin, Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan, Qinghua Zhang, Keith Lindsey, Xianlong Zhang. Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication. Nat Genet, 2017, 49(4): 579-587

7. 胡海燕, 刘迪秋, 李允静, 李阳, 涂礼莉*, 一个棉花纤维伸长期优势表达启动子pGhFLA1 的克隆与鉴定, 作物学报, 2017, 43(6): 849-854

2016年

8. Kai Guo, Lili Tu*, Pengcheng Wang, Xueqiong Du, Shue Ye, Ming Luo and Xianlong Zhang, 2016, Ascorbate alleviates Fe deficiency-induced stress in cotton (Gossypium hirsutum) by modulating ABA levels. 2016. Frontiers in Plant Science, doi: 10.3389/fpls.2016.01997

9. Kai Guo, Xueqiong Du, Lili Tu*, Wenxin Tang, Pengcheng Wang, Maojun Wang, Zhen Liu, Xianlong Zhang, 2016, Fibre elongation requires normal redox homeostasis modulated by cytosolic ascorbate peroxidase in cotton (Gossypium hirsutum). J Exp Bot, 67(11):3289-3301

10. Maojun Wang, Pengcheng Wang, Lili Tu , Sitao Zhu, Lin Zhang, Zhonghua Li, Qinghua Zhang, Daojun Yuan and Xianlong Zhang *, 2016, Multi-omics maps of cotton fibre reveal epigenetic basis for staged single-cell differentiation. Nucleic Acids Res, 44(9):4067-4079

11. Haiyan Hu, Xin He, Lili Tu , Longfu Zhu, Sitao Zhu, Zhonghe Ge, Xianlong Zhang *. GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like. Plant J, 2016，88(6):921-935

12. Yang Li, Lili Tu*, Filomena A Pettolino, Shengmei Ji, Juan Hao, Daojun Yuan, Fenglin Deng, Jiafu Tan, Haiyan Hu, Qing Wang, Danny J. Llewellyn, Xianlong Zhang, 2016, GbEXPATR, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring. Plant Biotechnol J, 14(3): 951–963

2015年

13. Yang Li, Lili Tu*, Zhengxiu Ye, Maojun Wang, Wenhui Gao, Xianlong Zhang, 2015, A cotton fiber-preferential promoter, PGbEXPA2, is regulated by GA and ABA in Arabidopsis. Plant Cell Rep, 34(9):1539-49

14. Maojun Wang, Daojun Yuan, Lili Tu , Wenhui Gao, Yonghui He, Haiyan Hu, Pengcheng Wang, Nian Liu, Keith Lindsey, Xianlong Zhang *. Long noncoding RNAs and their proposed functions in fibre development of cotton (Gossypium spp.). New Phytol, 2015, 207(4):1181-1197

15. Daojun Yuan, Zhonghui Tang, Maojun Wang, Wenhui Gao, Lili Tu , Xin Jin, Lingling Chen, Yonghui He, Lin Zhang, Longfu Zhu, Yang Li, Qiqi Liang, Zhongxu Lin, Xiyan Yang, Nian Liu, Shuangxia Jin, Yang Lei, Yuanhao Ding, Guoliang Li, Xiaoan Ruan, Yijun Ruan*, Xianlong Zhang *, 2015, The genome sequence of Sea-Island cotton (Gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres. Sci Rep, 5: 17662, doi: 10.1038/srep17662

2014年

16. 涂礼莉, 谭家福, 郭凯, 李中华, 张献龙*, 2014, 类黄酮代谢途径与棉花纤维发育, 中国科学(生命科学) Vol. 44 Issue (8): 758-765 （综述）

17. Nian Liu, Lili Tu, Wenxin Tang, Wenhui Gao, Keith Lindsey, Xianlong Zhang*, 2014, Small RNA and degradome profiling reveals a role for miRNAs and their targets in the developing fibers of Gossypium barbadense. The Plant J, 80(2):331-44, DOI: 10.1111/tpj.12636

18. Juan Hao, Sheng Chen, Lili Tu*, Haiyan Hu, Xianlong Zhang, 2014, GhH2A12, a replication-dependent histone H2A gene from Gossypium hirsutum, is negatively involved in development of cotton fiber cells. Plant Cell Reports 33: 1711-21

19. Wenxin Tang, Yonghui He, Lili Tu*, Maojun Wang, Yang Li, Yong-Ling Ruan, Xianlong Zhang. 2014, Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex. Plant Mol Biol, 85(6):613-25

20. Jie Han, Jiafu Tan, Lili Tu*, Xianlong Zhang, 2014, A peptide hormone gene, GhPSK promotes fiber elongation and contributes to longer and finer cotton fiber, Plant Biotechnol J, 12: 861-871

21. Wenxin Tang, Lili Tu*, Xiyan Yang, Jiafu Tan, Fenglin Deng, Juan Hao, Kai Guo, Keith Lindsey, Xianlong Zhang* , 2014， The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating ROS production, New Phytologist, 202(2): 509-20

2013年及以前

22. Jiafu Tan, Maojun Wang, Lili Tu*, Yichun Nie, Yongjun Lin and Xianlong Zhang, 2013, The Flavonoid Pathway Regulates the Petal Colors of Cotton Flower. PLoS ONE 8(8): e72364. doi:10.1371/journal.pone.0072364

23. Jiafu Tan, Lili Tu, Fenglin Deng, Haiyan Hu, Yichun Nie, and Xianlong Zhang*, 2013, A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin, Plant Physiology, 162: 86-95

24. Juan Hao, Lili Tu, Haiyan Hu, Jiafu Tan, Fenglin Deng, Wenxin Tang, Yichun Nie and Xianlong Zhang*, 2012, GbTCP, a cotton TCP transcription factor, confers fibre elongation and root hair development by a complex regulating system. J Exp Bot, 63: 6267-6281

25. Fenglin Deng, Lili Tu, Jiafu Tan, Yang Li, Yichun Nie, Xianlong Zhang, 2012, GbPDF1 (Protodermal factor 1) is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2. Plant Physiology, 158: 890–904

26. Jiafu Tan, Lili Tu*, Fenglin Deng, Rui Wu, Xianlong Zhang, 2012, Exogenous Jasmonic Acid Inhibited Cotton Fiber Elongation. The Journal of Plant Growth Regulation, 31: 599–605

27. Yuan Daojun, Lili Tu, and Zhang Xianlong*. Generation, Annotation and Analysis of First Large-Scale Expressed Sequence Tags from Developing Fiber of Gossypium barbadense L. PLoS ONE, 2011,6(7):e22758

28. M. Farooq Hussain Munis, Lili Tu, Fenglin Deng, Jiafu Tan, Li Xu, Shicheng Xu, Lu Long and Xianlong Zhang*, 2010, A thaumatin-like protein (PR-5) gene of cotton (Gossypium barbadence L.) involved in fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco,Biochemical and Biophysical Research Communications，393: 38-44

29. Yunjing Li, Diqiu Liu, Lili Tu, Xianlong Zhang*, Li Wang, Longfu Zhu, Jiafu Tan, Fenglin Deng，2010，Suppression of GhAGP4 gene expression repressed the initiation and elongation of cotton fiber，Plant Cell Reports, 29: 193-202

30. Diqiu Liu, Lili Tu, Yunjing Li, Li Wang, Longfu Zhu, Xianlong Zhang*, 2008, Genes encoding Fasciclin-Like Arabinogalactan proteins are specifically expressed during cotton fiber development, Plant Mol Biol Rep, 26: 98-113

31. Diqiu Liu, Lili Tu, Li Wang, Yunjing Li, Longfu Zhu, Xianlong Zhang*, Characterization and expression of plasma and tonoplast membrane aquaporins in elongating cotton fibers, Plant Cell Rep, 2008, 27: 1385–1394

32. Lili Tu, Xian-Long Zhang*, Shao-Guang Liang, Di-Qiu Liu, Long-Fu Zhu, Fan-Chang Zeng, Yi-Chun Nie, Xiao-Ping Guo, Feng-Lin Deng, Jia-Fu Tan, Li Xu, 2007, Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development, Plant Cell Reports, 26: 1309-1320

33. Lili Tu, Xianlong Zhang*, Diqiu Liu, Shuangxia Jin, Jinglin Cao, Longfu Zhu, Fenglin Deng, Jiafu Tan, Cunbin Zhang, 2007, Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis, Chinese Science Bulletin 52: 3110-3117

34. Diqiu Liu, Xianlong Zhang*, Lili Tu, Longfu Zhu, Xiaoping Guo, 2006, Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and later fiber development stages in cotton, Molecular Biology, 40(5): 741-749

35. Zhu Long-Fu, Tu Li-Li , Zhen Fan-Chang, Liu Di-Qiu, Zhang Xian-Long*, 2005, An Improved Simple Protocol for Isolation of High Quality RNA from Gossypium spp. Suitable for cDNA Library Construction, 作物学报, 31(12)：1657-1659

36. 涂礼莉，张献龙*等，2003，海岛棉NBS类型抗病基因类似物的起源、多样性及进化，遗传学报，30：1071-1077