学习经历

2005.09-2009.06，西北大学，生物科学专业本科学习，获理学学士学位；

2009.09-2014.06，山东大学，生物化学与分子生物学专业硕博连读，获理学博士学位

工作经历

2014.07 -至今，yl6809永利yl6809永利植物保护系，从事教学科研工作，专注于昆虫发育可塑性（滞育）的内分泌机制研究，创新害虫绿色防控策略，服务于区域蔬菜的健康安全生产。

研究内容简述

多种温带昆虫在长期进化过程中形成了滞育特性，即在不良环境（高温，低温，干旱，事物缺乏等）到来之前，可预先编程从而进入一种发育停滞的状态，类似于动物休眠或冬眠。滞育昆虫常表现出发育停滞、代谢降低、脂肪堆积、衰老延缓、寿命延长，因此可作为代谢调控，动物肥胖、衰老和长寿的研究模型。在农业生态系统中，多种害虫可利用其滞育特性有力规避不良环境，保证种群延续，进而成灾。因此，通过人工干预滞育可将害虫暴露于不利环境中，进而诱导害虫“生态自杀”。另外，通过调控天敌昆虫滞育，可有效延长天敌货架期，优化天敌田间使用策略。

基于以上考虑，我们以具生殖滞育特性的农业害虫和天敌昆虫为材料，整合传统生理学、现代分子生物学和细胞生物学等方法和技术，试图解析1）昆虫在滞育诱导期如何感受环境因子光周期、2）光周期如何调控内分泌系统进而决定昆虫滞育，3）内分泌信号如何调控滞育生理。通过回答这些问题，我们将逐步解析昆虫滞育调控的关键基因和通路，为基于滞育调节进而控制害虫成灾的绿色防控策略提供基因靶标，同时从比较生理学的角度为动物、乃至人类肥胖、衰老和长寿研究提供参考。

2. 异色瓢虫生殖滞育准备中体内低水平保幼激素的维持机制，国家自然科学基金，2018-2020，参加

3. JH-Met信号调控大猿叶虫卵黄发生关键基因转录的机制研究，国家自然科学基金，2016-2018，主持

4. 大猿叶虫幼虫期对光周期滞育诱导的分子应答，国家自然科学基金，2016-2019，参加

5. ILPs调控大猿叶甲滞育准备期营养吸收和能量储存的分子机制，yl6809永利新进博士科研启动专项，2014-2015，主持

#Equal contribution, *Co-responding author

以第一或通讯作者发表的论文

1. Liu W(刘文)#, Tan Q-Q#, Zhu L, Li Y, Zhu F, Lei CL, Wang X-P*: Absence of juvenile hormone signalling regulates the dynamic expression profiles of nutritional metabolism genes during diapause preparation in the cabbage beetle Colaphellus bowringi. Insect Molecular Biology 2017, 26(5):530-542.

2. Zhu L, Yin T-Y, Sun D, Liu W(刘文)*, Zhu F, Lei C-L, Wang X-P: Juvenile hormone regulates the differential expression of putative juvenile hormone esterases via methoprene-tolerant in non-diapause-destined and diapause-destined adult female beetle. Gene 2017, 627:373-378.

3. Zhu L, Liu W(刘文)*, Tan Q-Q, Lei C-L, Wang X-P: Differential expression of circadian clock genes in two strains of beetles reveals candidates related to photoperiodic induction of summer diapause. Gene 2017, 603:9-14.

4. Tan Q-Q, Liu W(刘文)*, Zhu F, Lei C-L, Wang X-P*: Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression. Scientific Reports 2017, 7:40509.

5. 周睿琦，谈倩倩，王小平，刘文*: 大猿叶虫滞育品系和非滞育品系交配行为的比较. 环境昆虫学报 2017, 39(1): 167－171.

6. Liu W(刘文), Li Y, Zhu L, Zhu F, Lei C-L, Wang X-P*: Juvenile hormone facilitates the antagonism between adult reproduction and diapause through the methoprene-tolerant gene in the female Colaphellus bowringi. Insect Biochemistry and Molecular Biology 2016, 74:50-60.

7. Liu W(刘文), Li Y, Guo S, Yin H, Lei C-L, Wang X-P*: Association between gut microbiota and diapause preparation in the cabbage beetle: a new perspective for studying insect diapause. Scientific Reports 2016, 6:38900.

8. Liu W(刘文), Cai M-J, Zheng C-C, Wang J-X, Zhao X-F*: Phospholipase cγ1 connects the cell membrane pathway to the nuclear receptor pathway in insect steroid hormone signaling. Journal of Biological Chemistry 2014, 289(19):13026-13041.

9. Liu W(刘文), Cai M-J, Wang J-X, Zhao X-F*: In a nongenomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription. Endocrinology 2014, 155(5):1738-1750.

10. Liu W(刘文), Zhang F-X, Cai M-J, Zhao W-L, Li X-R, Wang J-X, Zhao X-F*: The hormone-dependent function of Hsp90 in the crosstalk between 20-hydroxyecdysone and juvenile hormone signaling pathways in insects is determined by differential phosphorylation and protein interactions. Biochim Biophys Acta 2013, 1830(11):5184–5192.

合作发表的论文

1. Chen C-H, Pan J, Di YQ, Liu W(刘文), Hou L, Wang J-X, Zhao X-F*: Protein kinase C delta phosphorylates ecdysone receptor B1 to promote gene expression and apoptosis under 20-hydroxyecdysone regulation. Proc Natl Acad Sci U S A 2017, 114(34):E7121-E7130.

2. Quan W-L, Liu W(刘文), Zhou R-Q, Chen R, Ma W-H, Lei C-L, Wang X-P*: Difference in diel mating time contributes to assortative mating between host plant-associated populations of Chilo suppressalis. Scientific Reports 2017, 7.

3. Tan Q-Q, Liu W(刘文), Zhu F, Lei C-L, Hahn DA*, Wang X-P*: Describing the Diapause-Preparatory Proteome of the Beetle Colaphellus bowringi and Identifying Candidates Affecting Lipid Accumulation Using Isobaric Tags for Mass Spectrometry-Based Proteome Quantification (iTRAQ). Frontiers in Physiology 2017, 8(251).

4. Tan Q-Q, Zhou R-Q, Liu W(刘文), Feng L, Wang X-P*: Biological characteristics of a non-photoperiodic-diapause strain of the cabbage beetle Colaphellus bowringi (Coleoptera: Chrysomelidae). Entomological Science 2017, 20(1):50-56.

5. Quan W-L, Liu W(刘文), Zhou R-Q, Qureshi SR, Ding N, Ma W-H, Lei C-L, Wang X-P*: Do differences in life-history traits and the timing of peak mating activity between host-associated populations of Chilo suppressalis have a genetic basis? Ecology and Evolution 2016, 6(13):4478-4487.

6. Tan Q-Q, Feng L, Liu W(刘文), Zhu L, Lei C-L, Wang X-P*: Differences in the pre-diapause and pre-oviposition accumulation of critical nutrients in adult females of the beetle Colaphellus bowringi. Entomologia Experimentalis et Applicata 2016, 160(2):117-125.

7. Jing Y-P, Liu W(刘文), Wang J-X, Zhao X-F*: The steroid hormone 20-hydroxyecdysone via nongenomic pathway activates Ca2+/calmodulin-dependent protein kinase II to regulate gene expression. Journal of Biological Chemistry 2015, 290(13):8469-8481.

8. Dong D-J, Jing Y-P, Liu W(刘文), Wang J-X, Zhao X-F*: The Steroid Hormone 20-Hydroxyecdysone Up-regulates Ste-20 Family Serine/Threonine Kinase Hippo to Induce Programmed Cell Death. Journal of Biological Chemistry 2015, 290(41):24738-24746.

9. Tan Q-Q, Zhu L, Li Y, Liu W(刘文), Ma W-H*, Lei C-L, Wang X-P*: A de novo transcriptome and valid reference renes for quantitative Real-Time PCR in Colaphellus bowringi. PLoS One 2015, 10(2):e0118693.

10. Cai M-J, Liu W(刘文), Pei X-Y, Li X-R, He H-J, Wang J-X, Zhao X-F*: Juvenile hormone prevents 20-hydroxyecdysone-induced metamorphosis by regulating the phosphorylation of a newly identified broad protein. Journal of Biological Chemistry 2014, 289(38):26630-26641.

11. Cai M-J, Dong D-J, Wang Y, Liu P-C, Liu W(刘文), Wang J-X, Zhao X-F*: G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane. Cell Communication and Signaling 2014, 12(1):9.

12. Zhao W-L, Liu C-Y, Liu W(刘文), Wang D, Wang J-X, Zhao X-F*: Methoprene-tolerant 1 regulates gene transcription to maintain insect larval status. Journal of Molecular Endocrinology 2014, 53(1):93–104.

13. Ren J, Li X-R, Liu P-C, Cai M-J, Liu W(刘文), Wang J-X, Zhao X-F*: G-protein alphaq participates in the steroid hormone 20-hydroxyecdysone nongenomic signal transduction. Journal of Steroid Biochemistry and Molecular Biology 2014, 144PB:313-323.

14. Dong D-J, Liu W(刘文), Cai M-J, Wang J-X, Zhao X-F*: Steroid hormone 20-hydroxyecdysone regulation of the very-high-density lipoprotein (VHDL) receptor phosphorylation for VHDL uptake. Insect Biochemistry and Molecular Biology 2013, 43(4):328-335.

15. Liu C-Y, Liu W(刘文), Zhao W-L, Wang J-X, Zhao X-F*: Upregulation of the expression of prodeath serine/threonine protein kinase for programmed cell death by steroid hormone 20-hydroxyecdysone. Apoptosis 2013, 18(2):171-187.

16. Hou L, Cai M-J, Liu W(刘文), Song Q, Zhao X-F*: Small GTPase Rab4b participates in the gene transcription of 20-hydroxyecdysone and insulin pathways to regulate glycogen level and metamorphosis. Developmental Biology 2012, 371(1):13-22.

17. Cai M-J, Liu W(刘文), He H-J, Wang J-X, Zhao X-F*: Mod(mdg4) participates in hormonally regulated midgut programmed cell death during metamorphosis. Apoptosis 2012, 17(12):1327-1339.